The results of a phylogenetic analysis of the 16s rrna gene indicated that these strains belong to the genus desulfotomaculum, but are distinct from previously described desulfotomaculum species. Physical characteristics light coloured, free flowing powder. Genome analyses of the carboxydotrophic sulfatereducers. Conversely, extracts ofdesulfovibrio gigas, desulfovibrio vulgaris, anddesul. They are phylogenetically very closely related and belong to subgroup a of the desulfotomaculum cluster 1.
Extracts of desulfotomaculum nigrificans, desulfotomaculum orientis, and desulfotomaculum ruminis exhibit lowlevels ofinorganic pyrophosphatasebut were found to have high levels of pyrophosphate. The efficacy of the bark of eucalyptus tereticornis smith as an adsorbent for the removal of metal ions and sulphate from acid mine water was assessed. It is not clear as yet whether the tetrapyrrolic prosthetic group of desulfoviridin is present in the enzyme as the free. The results of phylogenetic analysis demonstrated that the genus desulfotomaculum already has lost the clustering monophyly and was segregated into some distinct groups with low sequence similarity. It reacted with na2 s2 o4 and was characterized by absorption maxima in the oxidized state at 392, 582, and 700 nm. Alternative procedures for enumeration of desulfotomaculum. A genus of rodshaped straight or curved, anaerobic, chemoorganotrophic motile bacteria that stain gramnegative but have grampositive cell walls. These include anaerobic bacteria that reduce sulfates srb, sulfatereducing bacteria 88,94,95, as desulfovibrio vulgaris, desulfuricans, fairfieldensis, gigas or desulfotomaculum nigrificans. The type species of the genus is desulfotomaculum nigrificans approved lists 1980 emend.
A summary of the diversity and biochemistry of bacteria that carry out a dissimilatory sulfate reduction has been given in chapter 24. This digital document is a journal article from chemosphere, published by elsevier in. Desulfitation article about desulfitation by the free. Read alternative procedures for enumeration of desulfotomaculum nigrificans spores in raw ingredients of soy protein.
Desulfotomaculum nigrificans atcc 19998 were kindly supplied byj. Biocorrosion of dental alloys due to desulfotomaculum. Desulfotomaculum acetoxidans atcc 49208 designation. It reacted with na 2 s 2 o 4 and was characterized by absorption maxima in the oxidized state at 392, 582, and 700 nm. Sodium sulfite, upon reduction, produces hydrogen sulfide. Desulfotomaculum nigrificans definition of desulfotomaculum. The results of phylogenetic analysis demonstrated that the genus desulfotomaculum already has lost the clustering monophyly and was segregated into.
Four other species were sensitive to 3 h of aeration. Desulfotomaculum species are common in anoxic freshwater, brackish or marine sediments. Desulfotomaculum nigrificans werkman and weaver 1927 campbell and postgate 1965 approved lists 1980 emend. Six strains of thermophilic, endosporeforming, sulfatereducing bacteria were enriched and isolated from 2. Desulfotomaculum definition of desulfotomaculum by.
Biotechnology to atcc valued customers, atcc stands ready to support our customers needs during the coronavirus pandemic. This is just one example of how critical the trna is to defining codon meaning. Desulfotomaculum nigrificans werkman and weaver campbell and postgate atcc 19858 freezedried forprofit. In 1965, the genus desulfotomaculum was created for sulfatereducing bacteria that form heatresistant spores. The first isolate of this genus, a moderate thermophile, was originally described as clostridium nigrificans werkman and weaver, 1927, later as sporovibrio desulfuricans starkey, 1938, and finally. Bioremediation of acid mine water using fly ash and.
About 96% of fe, 75% of zn, 92% of cu and 41% of sulphate removal was achieved from the acid mine water of ph 2. Relatively few nucleotide changes in a trna can lead to. Pdf description of desulfotomaculum nigrificans subsp. Later, starkey 1938 renamed it to sporovibrio desulfuricans before it was finally. Microbiologicallyenhanced galvanic corrosion of the steel. Theflask was connected to an absorption train of two test tubes, eachcontaining. Review of desulfotomaculum species and proposal of the genera. In the presence of na 2 s 2 o 4, p582 formed complexes with co and, under alkaline conditions, pyridine. Desulfotomaculum belongs in the clostridia class of the firmicutes. The cluster consists of phylogenetically wellseparated lineages containing i desulfotomaculum nigrificans, desulfotomaculum aeronauticum, and desulfotomaculum ruminis, ii desulfotomaculum. Sulfatereducing bacteria that form heatresistant endospores are classified in one genus, desulfotomaculum campbell and postgate, 1965. Desulfotomaculum arcticum is a sporeforming, moderately thermophilic, sulfatereducing bacterium. Formation of spores resulted in oxygen resistance of d.
The results show that the srb enhance the generation of a galvanic coupling effect between bare steel and the steel under. In thiosulfate reductase isolated from desulfotomaculum. The separation of an autoxidizable brown pigment, p582, from desulfotomaculum nigrificans is described. Doelle, in bacterial metabolism second edition, 1975. Growth and antioxidant activity of desulfotomaculum. If the sporeforming sulfatereducing bacteria are separated from the nonsporulating types, the specific epithet desulfuricans must remain with the nonsporulating group, and so must the generic name desulfovibrio, since the latter is the present equivalent of beijerincks spirillum. Desulfotomaculum definition of desulfotomaculum by medical.
Carbon monoxidereacting pigment from desulfotomaculum nigrificans and its possible relevance to sulfite reduction. This type of spoilage is associated with anaerobic sulphidereducing spore forming bacteria such as the themophilic desulfotomaculum nigrificans and also the mesophilic clostridium sporogenes. Desulfotomaculum article about desulfotomaculum by the. In this work, microbiologicallyenhanced galvanic corrosion between the carbon steel beneath a deposit and bare steel was investigated in simulated oilfieldproduced water containing sulfatereducing bacteria srb, in this case desulfotomaculum nigrificans. Draft genome sequence of the sulfatereducing bacterium. Desulfotomaculum copahuensis strain cindefi1 is a novel sporeforming sulfatereducing bacterium isolated from the copahue volcano area, argentina. Here, we present its draft genome in which we found genes related with the anaerobic respiration of sulfur compounds similar to those present in the copahue environment. Desulfitation the removal from uncooked preserved fruit and vegetables of the sulfurous acid used in sulfitation.
Desulfotomaculum acetoxidans widdel and pfennig atcc 49208. One of the first species that was included in this new genus was. Based products, journal of food science on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at. Jan 01, 2009 the efficacy of the bark of eucalyptus tereticornis smith as an adsorbent for the removal of metal ions and sulphate from acid mine water was assessed. The addition of acetate enhanced growth and thiosulfate disproportionation. The genus desulfotomaculum is a heterogeneous group of sporeforming sulfatereducing bacteria. The addition of acetate enhanced growth and thiosulfate disproportionation by d. Desulfotomaculum thermobenzoicum, but not desulfotomaculum nigrificans, desulfotomaculum ruminis, or desulfosporosinus orientis, grew by disproportionation of thiosulfate, forming stoichiometric amounts of sulfate and sulfide. Cell free extracts were prepared by suspending the wet cell paste in 0.
Desulfotomaculum nigrificans derived from atcc 7946, sku. Carbon monoxidereacting pigment from desulfotomaculum. Comparative bioenergetics sulfate reduction desulfovibrio. Organic nutrition stimulation of growth or sulfide yield, or both, by materials such as yeast extract or peptone hasbeenreported often 19, 38, 43, 119, 4. The authors characterized a novel trnaseclike trnacys from desulfotomaculum nigrificans that recodes uga codons with cysteine instead of selenocysteine and dubbed this new molecule trnarec to indicate its ability to recode with cysteine. Thus, we propose a new species, desulfotomaculum putei, for them, with strain th11 smcc w459 as the type strain. Jul 20, 2018 the type species of the genus is desulfotomaculum nigrificans approved lists 1980 emend. The phosphoroclastic system used in this study was prepared essentially as reported for obtaining the ferredoxin free clastic system from clostridium pasteurianum. Taken into account the absence of direct contacts of the city buildings with groundwater and low winter temperatures, the role of nonsporeforming and sporeforming but thermophilic sulfatereducing bacteria genus desulfotomaculum in destruction of houses is minimal. Desulfotomaculum wikispecies, free species directory. Desulfotomaculum nigrificans werkman and weaver 1927 campbell and postgate 1965, 361 al clostridium nigrificans werkman and weaver 1927, 63. To atcc valued customers, atcc stands ready to support our customers needs during the coronavirus pandemic.
Desulfotomaculum is a genus of grampositive, obligately anaerobic soil bacteria. Reader is wrong desulfotomaculum belongs in the clostridia. Desulfotomaculum nigrificans atcc 19858 designation. Pdf phylogenetic analysis of the genus desulfotomaculum. The first isolate of this genus, a moderate thermophile, was originally described as clostridium. Sporulation was observed only when acetate was the organic substrate. Half volume of the inoculum was used immediately growth and antioxidant activity of desulfotomaculum acetoxidans dsm 771 cultivated in acetate or lactate. Desulfotomaculum nigrificans werkman and weaver campbell. Thiosulfate disproportionation by desulfotomaculum. Iron sulphite agar oxoid cm79 formula tryptone grams per litre 10.
Desulfotomaculum acetoxidans widdel and pfennig atcc. Its type strain is 15 t dsm 17038 t jcm 12923 t references. Bioremediation studies using a typical sulfate reducing bacterium namely desulfotomaculum nigrificans, after pretreatment of the. Desulfotomaculum nigrificans derived from atcc 7946.
Although the majority of the sulfatereducing bacteria are unable to utilize glucose, desulfotomaculum nigrificans 37, which is a thermophilic member of the sulfatereducing bacteria 36, can be adapted to utilize glucose as an energy source 5. Desulfotomaculum thermocisternum desulfotomaculum aeronauticum desulfotomaculum halophilum desulfotomaculum kuznetsovii. The desulfitation is performed in such a way that the semifinished and finished products retain their original natural qualities color, odor, food value, and taste. Desulfotomaculum article about desulfotomaculum by the free. The results of phylogenetic analysis demonstrated that the genus desulfotomaculum already has lost the clustering monophyly and was segregated into some distinct groups with low sequence. Based products, journal of food science on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Siroheme and sirohydrochlorin journal of biological. Cells of strain gss09t were anaerobic, gramstainpositive, motile, endosporeforming and rodshaped. Reducing agents did not protect the vegetative cells of this. Separation of hydrogenase from intact cells of desulfovibrio vulgaris. The cells of these strains were motile rods that were 1 to 1. The studies of bacterial proliferation and an analysis of the chemical composition of sample surfaces were conducted. Survival of sulfatereducing bacteria after oxygen stress. Review of desulfotomaculum species and proposal of the. Acetateoxidizing sulfatereducing bacteria of the desulfotomaculum acetoxidans type have been enriched from animal manure, rumen content and dung contaminated freshwater habitats, indicating that they are primarily intestinal bacteria. Desulfotomaculum ruminis desulfotomaculum nigrificans desulfotomaculum australicum desulfotomaculum thermobenzoicum desulfotomaculum geothermicum desulfotomaculum sp. The cells grew by oxidizing h2, formate, methanol weakly, lactate incompletely, to acetate and co2. Studies on removal of metal ions and sulphate reduction. Akagi, anddesulfotomaculum orientis atcc 19365 was purchased from the. Since the tetrapyrrolic prosthetic groups of the sulfite reductases of e.
Desulfotomaculum are usually found living in underground water, inside sediment or rock, or within feces of animals mainly pigs where little oxygen has reached. Desulfotomaculum nigrificans 4, a thermophilic sulfatereducing bacterium 3, in contrast to other sulfatereducers, can be cultivated in media containing glucose as the energy source. Studies on removal of metal ions and sulphate reduction using rice husk and desulfotomaculum nigrificans with reference to remediation of acid mine drainage an article from. Very limited knowledge is available on the potential mechanisms of metal reduction among gram. Sporulation and further nutritional characteristics of. One of the first species that was included in this new genus was d. Description of two new thermophilic desulfotomaculum spp. A novel dissimilatory feiiireducing bacterium, designated strain gss09t, was isolated from a compost sample by using a solid medium containing acetate and ferrihydrite as electron donor and electron acceptor, respectively. Comparative bioenergetics sulfate reduction desulfovibrio and. Desulfotomaculum nigrificans are moderately thermophilic members of the polyphyletic sporeforming genus desulfotomaculum in the family peptococcaceae. A type of sulfatereducing bacteria, desulfotomaculum can cause food spoilage in poorly processed canned foods. Our first job is to listen to and observe what our customers need, and meet those needs with quality products and services. The free energy of adsorption of the metal ions onto fly ash was found to be negative, while the adsorption process was endothermic and adhered to the lagergren pseudofirst order rate equation.
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